Neb phusion polymerase manual

2X Phusion PCR Master Mix contains 0. 04 UL Phusion HighFidelity DNA Polymerase, 2X Phusion HF Buffer (in F531) or 2X Phusion GC Buffer (in F532), and 400 M of each dNTP. Manufactured and qualitycontrolled at New England Biolabs, Thermo Scientific Phusion HighFidelity DNA Polymerase offers both high fidelity and robust performance. 50X higher fidelity than Taq; Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Instruction Manual Phusion HighFidelity PCR Kit POLYMERASES& AMPLIFICATION NEB# E0553SL reactions Phusion HighFidelity PCR Kit. 2 Kit Components: This kit contains a sufficient supply of polymerase, buffers, deoxynucleotides, Phusion DNA Polymerase last, in order to prevent any primer degradation caused The Phusion HighFidelity PCR Kit contains a sufficient supply of Phusion HighFidelity DNA Polymerase, Phusion HF and GC Buffers, deoxynucleotides, magnesium chloride, DMSO, and DNA size standard to perform 50 reactions (small) or 200 reactions (large).

RESEARCH Multiple Reassortment Between Pandemic (H1N1) 2009 manual on animal in uenza diagnosis and surveillance Reverse Transcriptase and Platinum Taq HiFi (Invitrogen, Carlsbad, CA, USA).

added to the DNA samples by Phusion DNA polymerase (NEB) before they were loaded on the illumina sequencer. Content Retrieval Phusion DNA Polymerase was developed by Finnzymes Oy, now a part of Thermo Fisher Scientific. This product is manufactured by New England Biolabs, Inc. under agreement with, and under the performance specifications of Thermo Fisher Scientific. Phusion DNA Polymerase per 50 L reaction volume gives good results, but the optimal amount can range from 0.

5 to 2 units per 50 L reaction depending on amplicon length and Phusion DNA Polymerase was developed by Finnzymes Oy, now a part of Thermo Fisher Scientific. This product is manufactured by New England Biolabs, Inc. under agreement with, and under the performance specifications of Thermo Fisher Scientific.

Phusion HighFidelity DNA Polymerase is comprised of a DNAbinding domain fused to a Pyrococcuslike proofreading polymerase. As a result of this unique functional pairing, Phusion DNA Polymerases are capable of generating PCR products with very high accuracy and speed. Use the NEB Tm Calculator to estimate an appropriate annealing temperature when using NEB PCR products.

Phusion HighFidelity DNA Polymerase offers Polymerase is a magnesium dependent enzyme. Excessive extreme performance for all major PCR Phusion DNA Polymerase possesses the following activities: 53 DNA polymerase activity and 35 exonuclease activity. It generates blunt ends in the amplification products. The Phusion HighFidelity PCR Kit contains a sufficient supply of Phusion HighFidelity DNA Polymerase, Phusion HF and GC Buffers, deoxynucleotides, magnesium chloride, DMSO, and DNA size standard to perform 50 reactions (small) or 200 reactions (large).



Phone: (819) 879-2705 x 9967

Email: [email protected]