Linearized vector for TOPO TA cloning of PCR products. Zero Blunt TOPO PCR Cloning Kits offers the fastest and easiest method for highefficiency (cloning of bluntend PCR products amplified with proofreading thermostable polymerases. The kits include linearized and topoisomerase Iactivated pCRBlunt IITOPO Vector for 5 Fiveminute, directional TOPO Cloning of bluntend PCR products into an entry vector Technology with Clonase II manual which is available for downloading from www.
invitrogen. com or by contacting Technical Service (see page 35). continued on next page. 2 Overview, continued pCRBlunt IITOPO Parental vector for TOPO cloning of blunt PCR products. Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. this vector is NOT available from Addgene please contact the manufacturer for further details. Plasmid: pCR blunt II TOPO.
This vector is NOT available from Addgene. This page is informational only. Please contact Zero Blunt TOPO PCR Cloning Kit Version K July 24, 2003 Zero Blunt TOPO PCR Cloning Kit Fiveminute cloning of bluntend PCR products Catalog Plasmid pCRBluntIITOPORhoC from Dr.
Richard Hynes's lab contains the insert Human RhoC and is published in Nature. 2000 Aug 3. 406(6795): 5325. This plasmid is available through Addgene.
pCR BluntII Topo Vector (Baylor) Library Method Overview A scalable method for targeted generation of cDNA clones to facilitate recovery of genes absent from the MGC collection was developed in the laboratory of Drs.
Richard Gibbs and John McPherson and is outlined below: pCRBlunt IITOPO Cloning Vector transOMIC technologies is the official archiving site for the Mammalian Gene Collection (MGC). This vector is one of the several expression and cloning vectors found in the MGC premier cDNA, Entry ORF and Lentiviral ORF collections.
4 Kit Contents and Storage Type of kits This manual is supplied with the following kits. Kit Quantity Cat. no. Zero Blunt PCR Cloning Kit pCRBluntIITOPO. Parental vector for TOPO cloning of blunt PCR products. Pcr blunt ii topo manual arts Blunt TOPO PCR Cloning Kit Quick Reference Instructions are provided to TOPO Clone your PCR product into pCRBlunt IITOPO and transform the reaction into chemically competent E. coli cells. Refer to the Zero Blunt TOPO PCR Cloning Kit manual Ligation of a bluntend PCR product disrupts expression of the lacZaccdB gene fusion permitting growth of only positive recombinants upon transformation.
Cells Invitrogen Zero Blunt TOPO PCR Cloning Kit, with pCRBlunt IITOPO Vector, One Shot TOP10 Chemically Competent E. coli, and PureLink Quick Plasmid Miniprep Kit 25 reactions Add 2 L of the TOPO Cloning reaction to a vial of One Shot E.
Refer to the Zero Blunt TOPO PCR Cloning Kit manual for a recipe for Low Salt LB medium. medium to the cells. 7. 9.