Agilent 3100 OFFGEL Fractionator User Manual Agilent Technologies 1 Product overview Intended Use 14 Features 16 Instrument and Accessories 17 This chapter gives an overview of the Agilent 3100 OFFGEL Fractionator. It describes the intended use and the fracti onation principles of the instrument, Agilent 3100 OFFGEL Fractionator pIbased fractionation of proteins and peptides with liquidphase recovery Achieve unprecedented sensitivity in LCMSbased proteomics experiments Sample complexity and large dynamic range of protein and peptide concentrations are major hurdles in the analysis of low abundance proteins.
Agilent 3100 OFFGEL Fractionator User Manual Agilent Technologies 1 Product overview Intended Use 12 Features 14 Instrument and Accessories 15 This chapter gives an overview of the Agilent 3100 OFFGEL Fractionator. It Agilent 3100 OFFGEL Fractionator User Manual Agilent Technologies. 1. The Agilent 3100 OFFGEL Fractionator is designed to increase the number of proteins and peptides that can be identified in a whole cell lysate by separating the peptides of interest by their isoelectric point (pI) prior to analysis by PAGE, western blots, mass spec, HPLC, etc.
etc. Agilent Technologies Agilent 3100 OFFGEL Fractionator Kit Quick Start Guide 3100 OFFGEL Fractionator right (see Figure 4 in Section 1 of the Agilent 3100 User Manual). 2 Remove the protective backing from the IPG strip gel. Agilent Technologies Agilent 3100 OFFGEL Fractionator Installation Quick Guide with Windows 7 For details refer to the 3100 OFFGEL user manual. NOTE. This address reflects the default IP address of the Agilent 3100 OFFGEL fractionator. It is different from the IP address of your PC.
Site is currently unavailable due to system maintenance activity. We apologize for the inconvenience and appreciate your patience. If you have questions or concerns, please contac Agilent 3100 OFFGEL Fractionator Prefractionation of proteins and peptides prior to LCMS analysis can dramatically increase the number of proteins ultimately identified.
The Agilent 3100 OFFGEL Fractionator uses a novel isoelectric focusing technique to achieve excellent pIbased fractionation. Offgel electrophoresis with the Agilent 3100 OFFGEL Fractionator provides protein and peptide separation with excellent resolution and easy, insolution recovery.
Agilent 3100 OFFGEL Fractionator Instructions for part numbers 3100 OFFGEL 12 Well Frame Set 3100 OFFGEL 24 Well Frame Set Set Checklist 12 Frames (12 wells or 24 wells) 12 Cover Seals (for 12 or 24 wells) Glycerol Solution Mineral Oil OFFGEL User Manual 67 4 68 Operation Performing a Fractionation OFFGEL User Manual Agilent 3100 OFFGEL Fractionator User Manual 5 Maintenance Cleaning the 3100 OFFGEL Fractionator 70 Firmware Update 76 This chapter describes cleaning procedures that should be used to do maintenance on your instrument at regular intervals or before every use.
In The Agilent 3100 OFFGEL Fractionator provides pIbased fractionation of peptides or proteins with exceptional resolution. Samples can be fractionated or highest resolution. Analysis of a protein IEF marker mix with the Agilent 2100 Bioanalyzer before and after OFFGEL electrophoresis (24 fractions, pH 310). The overlay of electropherograms